Ion Library Equalizer™ Kit

Catalog number:  4482298

Description:

The Ion Library Equalizer™ Kit is a simple and seamless bead-based solution replacing the need for library quantification and library dilutions for library normalization as required for any next generation sequencing workflow. The Ion Library Equalizer™ Kit helps reduce labor, reagent costs, and further simplifies the Ion semiconductor sequencing workflow for high sample-throughput or barcoded projects, as well as single or low sample number projects.

The kit workflow consists of three simple steps: 

1) Library amplification with Equalizer™ Primers
2) Library capture onto Equalizer™ Beads
3) Heat elution of the normalized library using a specially formulated Equalizer™ Elution Buffer

Simple Workflow 

• Seamlessly integrates into your existing library preparation workflow

• Reduced pipetting compared to the Agilent 2100 Bioanalyzer®-based workflow

• Faster workflow in high-throughput applications-no need for DNA quantification, calculations, and dilution of DNA libraries

• Minimizes library-to-library variability

Cost Effective 

• No additional capital equipment required

• Less expensive than Agilent 2100 Bioanalyzer®-based workflow (up to 10 samples) 

• Reduced AMPure® XP reagent usage compared to Agilent 2100 Bioanalyzer®-based workflow 

• Long shelf life: up to 18 months

• Convenient 96-reaction kit helps reduce ordering frequency

Flexible 

• Compatible with all Ion DNA library kits including Ion AmpliSeq™ DNA panels, Ion fragment library kits, and Ion Xpress™ Barcode adaptors

• Use with a single sample or in a 96-well plate format

• Compatible with 3rd party automated liquid handling systems for integrated library preparation and normalization

Includes: 
The Ion Library Equalizer Kit includes the necessary reagents to complete 96 normalization reactions. 

Required for Workflow: 

• Ion AmpliSeq™ Library Kit 2.0

• Ion AmpliSeq™ Ready-to-Use Panel or Custom Panel designed via the Ion AmpliSeq™ Designer (www.ampliseq.com) 

• --OR--

• Ion Plus Fragment Library Kit

• --OR--

• Ion Xpress™ Plus Fragment Library Kit

Optional: 
Ion Xpress™ Barcode Adapters Kits